Channel



Deep Insight
 
SLC5A8 and its role in tumorigenesis
 
Gene
 
ABCB5 (ATP-binding cassette, sub-family B (MDR/TAP), member 5)
 
AQP4 (aquaporin 4)
 
CLDN10 (claudin 10)
 
CLIC1 (chloride intracellular channel 1)
 
CYP4B1 (cytochrome P450, family 4, subfamily B, polypeptide 1)
 
DSG2 (desmoglein 2)
 
FXYD3 (FXYD domain containing ion transport regulator 3)
 
KCMF1 (potassium channel modulatory factor 1)
 
KCNH1 (potassium voltage-gated channel, subfamily H (eag-related), member 1)
 
MAP2 (microtubule-associated protein 2)
 
MYLK (myosin light chain kinase)
 
ORAI3 (ORAI calcium release-activated calcium modulator 3)
 
PERP (PERP, TP53 apoptosis effector)
 
PKD1 (polycystic kidney disease 1 (autosomal dominant))
 
PRSS8 (protease, serine, 8)
 
REST (RE1-silencing transcription factor)
 
S100A10 (S100 calcium binding protein A10)
 
SGK1 (serum/glucocorticoid regulated kinase 1)
 
SLC19A3 (solute carrier family 19 (thiamine transporter), member 3)
 
SLC4A1 (solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group))
 
SLC5A5 (solute carrier family 5 (sodium iodide symporter), member 5)
 
SLC5A8 (solute carrier family 5 member 8)
 
SLC9A3R1 (solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1)
 
STEAP1 (Six Transmembrane Epithelial Antigene of the Prostate 1)
 
TRPM1 (transient receptor potential cation channel, subfamily M, member 1)
 
TRPM8 (transient receptor potential cation channel, subfamily M, member 8)
 
TRPV1 (transient receptor potential cation channel, subfamily V, member 1)
 
TRPV2 (transient receptor potential cation channel, subfamily V, member 2)
 
TRPV6 (transient receptor potential cation channel, subfamily V, member 6)
 
VDAC1 (voltage-dependent anion channel 1)
 
A. SDS-PAGE of erythrocyte membrane protein. B. Schematic diagram of membrane skeleton proteins.
from : SLC4A1 (solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group))
 


The model of erythrocyte Cl-/HCO3- exchange.
from : SLC4A1 (solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group))
 


Schematic representation of TRPM8 protein (adapted from Latorre et al., 2011).

from : TRPM8 (transient receptor potential cation channel, subfamily M, member 8)
 


The canonical form comprises 764 aa (MW ~86 kDa) and is composed of six transmembrane spanning domains and a pore forming region between transmembrane domains 5 and 6. The N-terminal and C-terminal tails are in cytoplasmatic side. Six N-terminal ankyrin (ANK) repeats are present in N-terminal tail.

from : TRPV2 (transient receptor potential cation channel, subfamily V, member 2)
 


The four transmembrane, two extracellular, 3 helical and two cytoplasmic domains of claudin protein.

from : CLDN10 (claudin 10)
 


The diagram has been drawn following UniProtKB/Swiss-Prot database prediction and maintaining approximate length proportions among extracellular and intracellular segments. Transmembrane segments are represented by green rectangles, N-glycosylation sites in yellow.
from : SLC5A5 (solute carrier family 5 (sodium iodide symporter), member 5)
 


Diagram drawn following UniProtKB/Swiss-Prot database prediction and maintaining approximate length proportions among extracellular and intracellular segments. Transmembrane segments are represented by rectangles.
from : SLC5A8 (solute carrier family 5 member 8)
 


STEAP gene organization, mRNA transcripts and predicted protein structure. Green boxes correspond to exons.
from : STEAP1 (Six Transmembrane Epithelial Antigene of the Prostate 1)
 


Schematic representation of TRPV6 protein. Four subunits makes one channel pore. Several ankyrin repeats, one N-glycosylation site and several calmodulin binding sites (CaM) are indicated.
from : TRPV6 (transient receptor potential cation channel, subfamily V, member 6)
 


Representative MLCK protein isoforms shown with select structural/functional information, as compared with the full length isoform (nmMLCK1). The nonmuscle MLCK isoform variants (nmMLCK 1, 2, 3A, 3B) differ in the presence or absence of exons 11 and 30. All nmMLCK variants possess unique amino termini that are absent in the smooth muscle isoform, smMLCK, and two isoforms of telokin. The longer isoform of telokin, differing by one amino acid from its shorter version (with the aa 1790 deletion), is identical to the C-termini of nmMLCK and smMLCK isoforms shown (Watterson et al., 1999). Abbreviations: aa, amino acid; CaM, calmodulin; IgC2, immunoglobulin C-2 type domain; FN3, fibronectin type 3 domain. *Note: NCBI RefSeq NM_053030.2 (NP_444258.1) was permanently suppressed because there was insufficient support for the transcript and the CDS was partial.

from : MYLK (myosin light chain kinase)
 


from : KCMF1 (potassium channel modulatory factor 1)
 


Protein structure of polycystin-1 (PC1). The details of the protein domain structures are shown. Abreviation: GPS, GPCR Proteolystic Site; WSC, cell Wall integrity and Stress response Component; PLAT, (Polycystin-1, Lipoxygenase, Alpha-Toxin); REJ, Receptor for Egg Jelly. This graph was generated by using ExPASY Proteomics Server PROSITE module with some modifications.

from : PKD1 (polycystic kidney disease 1 (autosomal dominant))
 


Schematic illustrating REST protein and alternative isoforms. Uniprot.org shows the existence of 4 REST isoforms. Isoform 1 is the canonical sequence; it comprises two repression domains (RD1 and RD2), lysine-rich (400-603) and proline-rich (595-815) domains, a DNA binding domain (159-412) of eight zinc fingers (boxes), two nuclear localization signals (shown in red), a phosphodegron (E1009/S1013 or S1027/S1030) and a ninth C-terminal zinc finger (1006-1082). REST can recruit a variety of transcriptional co-repressors through interaction at RD1 or RD2; examples are illustrated including Sin3A (interaction maps to 32-122), Sin3B (43-57) and RCOR1 (1009-1087). Isoform 2 is truncated, retaining only zinc fingers 1-4 and localizes to the cytoplasm. Isoform 3, also called REST4 or sNRSF, is expressed in neuroblastoma and SCLC; it lacks RD2 and has a truncated DNA binding domain, but retains zinc fingers 1-5 and nuclear localization. Isoform 4 has selective deletion of zinc finger 5. (Palm et al., 1999; Coulson et al., 2000; Shimojo et al., 2001).
from : REST (RE1-silencing transcription factor)
 


from : S100A10 (S100 calcium binding protein A10)
 


Schematic representation of TRPV1 protein. Double broken line is representative of cellular membrane, transmembrane domains are numbered. Red spot indicates the position of the three ankyrin repeat domains and a representative image of the structural ankyrin repeat unit containing two antiparallel helices and a beta-hairpin, with repeats that are stacked in a superhelical arrangement is shows in black box (from NCBI Conserved Domains), N and C (-terminal domains). SP (signal peptide region), ANK (ankyrin regions, red box), TM (trasmembrane domain, grey box), ED (extracellular domain, blue box), CD (cytoplasmatic domain, orange box), PFD (pore forming domain, green box). An association domain (AD) in 685-713 region has been found necessary for self-association.
from : TRPV1 (transient receptor potential cation channel, subfamily V, member 1)
 


Contributor(s)
Written Sep 2020 Jean-Loup Huret
Citation
This paper should be referenced as such :
Huret JL. Channel. Atlas Genet Cytogenet Oncol Haematol. September 2020
URL : https://www.chromosomesincancer.eu/biocell/page.aspx?id=7